Skip to main content
Fig. 6 | BMC Genomics

Fig. 6

From: A systemic approach to screening high-throughput RT-qPCR data for a suitable set of reference circulating miRNAs

Fig. 6

We performed the validation of the chosen set of 13 miRNAs as suitable reference genes. Figures represent histograms of distributions of mean ranking of randomly selected 13 miRNAs (blue). Panels a and b show two validation sets attached in the Additional files and panel c shows data from a publicly available GSE109888. We sampled 13 random ones from the pool of miRNAs presented in a validation dataset 2000 times creating 2000 replicates of mean ranking of derived 3 miRNA combinations. This allowed to plot empirical distribution of mean ranking of combinations derived from any arbitrarily selected 13 miRNAs. Shown are mean rankings of single miRNAs (pink) and combinations of 3 miRNAs (blue). A red vertical line marks mean ranking of 3 miRNA combinations derived from the chosen set. The lower the average ranking the more suitable the combination to be a reference gene. Average ranking of combinations derived from the chosen set (the red vertical lines) was lower than 83.32, 84.76 and 97.45% of all average rankings in three validation sets, respectively. In summary, combination of 3 miRNAs picked from our set of 13 were repeatedly within top 15% of best normalizers in two datasets and significantly outperformed single-miRNA normalizers

Back to article page