Fig. 3

RT-PCR of genes expressed in Ae. aegypti spermathecae. Relative expression was determined in the spermathecae from virgin (Vir) or inseminated (Ins) females, and from the material collected in the spermathecal reservoir lumen (Cont) of inseminated females. Bar graphs show the fold-change of each sample normalized to S7 ribosomal gene. Reactions were done in triplicate using two biological replicates. Statistical analyses were performed using one-way ANOVA and Tukey’s multiple comparison test (α = 0.05). a: S7 (F = 1; R²: 0.25; P = 0.4219), b: Gld (F = 477.2; R²: 0.9907; P < 0.001; *P < 0.001; **P < 0.01), c: ChtB4 (F = 54.4; R²: 0.9236; P < 0.001; *P < 0.001; **P < 0.01), d: Atro-1 (F = 17.24; R²: 0.793; P = 0.0008; *P = 0.0031; **P = 0.0011), e: DHR4 (F = 29.27; R²: 0.8667; P = 0.0001. *P = 0.0003; **P = 0.0003), f: GALNT6 (F = 21.91; R²: 0.8296; P = 0.0003. *P = 0.0021; **P = 0.0004), g: ChtBD2 (F = 5.724; R²: 0.5599; P = 0.0249; *P = 0.0303), h: KSPI (F = 75.8; R²: 0.944; P < 0.0001. *P < 0.0001; **P < 0.0001), i: Na⁺/Ca²⁺ (F = 74.28; R²: 0.9429; P < 0.0001. *P < 0.0001; **P = 0.0009; ***P = 0.0003)