Table 3 Toxin transcripts recovered for Bothriechis nubestris and associated classifications as orthologs or paralogs, expected transcripts per million reads (TPM) estimated by RSEM, over expression classification as detected in intraspecific variation comparisons (i.e., above the 99th percentile of expected variance in expression based on a nontoxin null distribution), and coverage-based assessment of likely presence or absence

Bnubes-BPP-1

Ortholog

5097.77

63484.01

CLP1865

-

+

Bnubes-CRISP-1

Paralog

17682.06

8634.01

CLP1859

+

+

Bnubes-CTL-1

Ortholog

48790.4

45771.89

-

+

+

Bnubes-CTL-2

Ortholog

13469.89

9134.83

-

+

+

Bnubes-CTL-3

Ortholog

18273.91

8462.73

CLP1859

+

+

Bnubes-CTL-4

Paralog

134247.25

46096.08

CLP1859

+

+

Bnubes-CTL-5

Paralog

93992.03

44194.92

CLP1859

+

+

Bnubes-CTL-6

Paralog

41975.1

23098.03

CLP1859

+

+

Bnubes-HYAL-1

Ortholog

312.56

480.7

-

+

+

Bnubes-KUN-1

Paralog

211.54

231.29

-

+

+

Bnubes-LAAO-1

Ortholog

6946.11

12529.4

-

+

+

Bnubes-NGF-1

Ortholog

3347.21

5435.31

-

+

+

Bnubes-NUC-1

Ortholog

1184.42

2318.22

-

+

+

Bnubes-PDE-1

Ortholog

1156.15

1461.55

-

+

+

Bnubes-PLA₂-1

Ortholog

3073.31

3700.01

-

+

+

Bnubes-PLA₂-2

Ortholog

2321.73

209.08

CLP1859

+

+

Bnubes-PLA₂-3

Paralog

4646.1

91726.32

CLP1865

+

+

Bnubes-SVMPII-1

Ortholog

11115.83

7867.18

-

+

+

Bnubes-SVMPII-2

Ortholog

7446.39

7182.3

-

+

+

Bnubes-SVMPII-3

Ortholog

85.26

6966.13

CLP1865

+

+

Bnubes-SVMPII-4

Paralog

9408.28

9519.11

-

+

+

Bnubes-SVMPII-5

Paralog

72976.3

52932.86

-

+

+

Bnubes-SVMPIII-1

Ortholog

4.02

52.08

CLP1865

-

+

Bnubes-SVMPIII-2

Ortholog

7436.41

6075.36

-

+

+

Bnubes-SVMPIII-3

Ortholog

14334.66

14644.25

-

+

+

Bnubes-SVMPIII-4

Ortholog

6744.23

10192.23

-

+

+

Bnubes-SVMPIII-5

Ortholog

131295.22

69281.92

CLP1859

+

+

Bnubes-SVMPIII-6

Paralog

808.43

2990.11

-

+

+

Bnubes-SVSP-1

Ortholog

5793.23

2477.48

CLP1859

+

+

Bnubes-SVSP-2

Ortholog

1544.31

2924.28

-

+

+

Bnubes-SVSP-3

Ortholog

3126.56

3125.05

-

+

+

Bnubes-SVSP-4

Ortholog

7665.15

2252.2

CLP1859

+

+

Bnubes-SVSP-5

Ortholog

2301.11

4094.43

-

+

+

Bnubes-SVSP-6

Ortholog

5123.44

2684.33

-

+

+

Bnubes-SVSP-7

Ortholog

795.14

393.28

-

+

+

Bnubes-SVSP-8

Ortholog

3207.97

10487.13

-

+

+

Bnubes-SVSP-9

Paralog

823.13

475.48

-

+

+

Bnubes-VEGF-1

Ortholog

3542.02

413.99

CLP1859

+

+

Bnubes-VEGF-2

Ortholog

222.72

119.16

-

+

+

Bnubes-VEGF-3

Ortholog

109.03

51.22

-

+

+

Bnubes-VEGF-4

Ortholog

61.69

68.27

-

+

+

Bnubes-Waprin-1

Ortholog

28.73

25.35

-

+

+