Fig. 1
Heat map of Ct values of limited multiplex real time PCR data. Real time PCR results of set 1expressed as a heat map of Ct vales as a function of the spiked concentrations of different organisms. The intensity of green color scale represents Ct values; i.e., dark shades of green indicating lower Ct values. Grey boxes indicate ‘undetected’ (i.e., >Ct of 40) by real time PCR. Organism and corresponding strains are indicated across the top, condition and spiking concentrations (CFUs) (10 fold dilution steps 5 through 1) are along the right side (numbers in Table 2), and replicate number along the left side. Conditions are as follows: CB cocktail buffer, CF clean filter, and DF dirty filter. The x-axis indicates assay (or amplicon reference). The red and blue rectangles indicate false negative and positive results, respectively