Fig. 3

Heat map of sequence read counts from limited multiplex real time PCR reactions (48 h data). Amplicon sequence data represented as a heat map of read counts of set 1 amplicon sequencing on ONT platform (full 48 h of sequencing data presented). Expected assay results are presented in Table 4. The intensity of red indicates the number of read counts in log10 scale. Organism and corresponding strains are indicated across the top, condition and spiking concentrations (CFUs) (10 fold dilution steps 5 through 1) are along the right side (numbers in Table 2), and replicate number along the left side. Conditions are as follows: CB cocktail buffer, CF clean filter, and DF dirty filter