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Fig. 4 | BMC Genomics

Fig. 4

From: Comparison of the performance of an amplicon sequencing assay based on Oxford Nanopore technology to real-time PCR assays for detecting bacterial biodefense pathogens

Fig. 4

Graphical representation of read counts resulting from multiplex PCR reaction spiked with individual organisms. Read counts per assay per isolate sample during the first 6 h of sequencing are presented in log10 scale. Circles represent expected amplicon reference mapping (TP), crosses represent unexpected amplicon reference mapping (FP). Black dashed line represents an example read count threshold of 100 for True positive call, applied in Fig. 5b for demonstration purposes only

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