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Fig. 5 | BMC Genomics

Fig. 5

From: Comparison of the performance of an amplicon sequencing assay based on Oxford Nanopore technology to real-time PCR assays for detecting bacterial biodefense pathogens

Fig. 5

Heat map of sequence read counts from 14 plex real time PCR reactions (48 h data). Heat map of read counts resulting from sequencing of 14-plex PCR assay from single isolate spiked samples on ONT platform (48 h of sequencing data). a) Amplicon reference aligned read counts per assay per sample (no read count threshold applied for true positive call). b) Read count threshold of 100 applied (all read counts < 100 are reduced to zero, i.e. not called as a positive)

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BMC Genomics

ISSN: 1471-2164

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