Fig. 9

ONT sequencing PR curves. Curves are shown for each of the 14 assays tested (faceted by column), split over the 5 steps of the dilution series (faceted by rows). Replicates are kept separated for this analysis. The read count cutoff for calling positive/negative is log10 scaled. The area under the curve (AUC) is calculated using the ‘step’ method. Most assays achieve 100% precision and 100% recall near a read count cutoff of 100 for the highest 3 concentrations (3,4, and 5), and many achieve these perfect scores at the lowest 2 (1,2) with the exception of PRC_30