Table 1 Multiplex strategy for 513 total spiked samples
Set | Organism(s) | Strain | Dilution Steps | Matrices | Replicates | Total Barcodes | Flowcell# |
|---|---|---|---|---|---|---|---|
1 | Francisella tularensis | 239 | 5 | 3 | 3 | 45 | 1 |
Francisella tularensis | 240 | 5 | 3 | 3 | 45 | 2 | |
Francisella tularensis | 241 | 5 | 3 | 3 | 45 | 3 | |
Yersinia pestis | 113 | 5 | 3 | 3 | 45 | 4 | |
Yersinia pestis | 114 | 5 | 3 | 3 | 45 | 5 | |
Burkholderia mallei | 164 | 5 | 3 | 3 | 45 | 6 | |
Burkholderia pseudomallei | 197 | 5 | 3 | 3 | 45 | 7 | |
Bacillus anthracis | 708-gi | 5 | 3 | 3 | 45 | 8 | |
Bacillus anthracis | 708-live | 5 | 3 | 3 | 45 | 9 | |
2 | All agents (known, amplified) | all 9 | 4 | 3 | 3 | 36 | 10 |
All agents (blinded, amplified) | all 9 | 4 | 3 | 3 | 36 | 11 | |
All agents (known, unamplified) | all 9 | 4 | 3 | 3 | 36 | 12 |