Probe Dye Channel | Extraction 1 | Extraction 2 | Extraction 3 | Average Ct Set 2 | Average Ct Set 1* | Difference** |
---|
FAM | 22.56 | 24.00 | 24.46 | 23.67 | 28.02 | 3.56 |
VIC | 22.36 | 23.32 | 23.55 | 23.07 | 28.02 | 4.47 |
NED | 25.36 | 26.53 | 26.98 | 26.29 | 28.96 | 2.67 |
CY5 | 30.85 | 33.28 | 29.69 | 31.27 | 29.82 | −1.45 |
- Real-time PCR results of mixed agent samples using primer/probe cocktails. All 8 organism strains are included in the mixutre. *Averages of Ct values among each probe dye channel from Table 9. **Difference between mixed and individual probe results (average Set 1 Ct values minus average Set 2 Ct values)