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Table 10 Real-time PCR results of mixed agents and assays

From: Comparison of the performance of an amplicon sequencing assay based on Oxford Nanopore technology to real-time PCR assays for detecting bacterial biodefense pathogens

Probe Dye Channel

Extraction 1

Extraction 2

Extraction 3

Average Ct Set 2

Average Ct Set 1*

Difference**

FAM

22.56

24.00

24.46

23.67

28.02

3.56

VIC

22.36

23.32

23.55

23.07

28.02

4.47

NED

25.36

26.53

26.98

26.29

28.96

2.67

CY5

30.85

33.28

29.69

31.27

29.82

−1.45

  1. Real-time PCR results of mixed agent samples using primer/probe cocktails. All 8 organism strains are included in the mixutre. *Averages of Ct values among each probe dye channel from Table 9. **Difference between mixed and individual probe results (average Set 1 Ct values minus average Set 2 Ct values)