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Table 4 Summary of limited multiplex real-time PCR results for individual agents

From: Comparison of the performance of an amplicon sequencing assay based on Oxford Nanopore technology to real-time PCR assays for detecting bacterial biodefense pathogens

Organism

Strain

F. tularensis assays

Y. pestis assays

Burkholderia assays

B. anthracis assays

F. tularensis

239

122

78

TN

TN

240

116

TN

FN

TN

241

48

TN

TN

79

Y. pestis

113

75

76

TN

84

114

85

TN

FN

98

B. mallei

164

148

TN

TN

B. pseudomallei

197

FP

71

71

B. anthracis

708-gi

66

46

46

708

33

46

46

PCR Assay Number

23

28

29

30

9

11

14

15

49

50

65

01

04

07

Probe Dye / Channel

FAM

VIC

NED

CY5

FAM

VIC

NED

CY5

FAM

VIC

NED

FAM

VIC

NED

  1. Values in the cells indicate an observed positive result (Ct < 40) where a positive result was expected, and represent a PCR efficiency percentage. A minus sign (−) indicates the assay-organism combination was not tested. Cells containing FN or FP indicate an observed false negative or positive result, respectively. Cells containing TN indicate an observed negative or undetected result (Ct ≥ 40) where a positive result was not expected