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Table 8 Mapped read abundances per assay amplicon for each spiked organism

From: Comparison of the performance of an amplicon sequencing assay based on Oxford Nanopore technology to real-time PCR assays for detecting bacterial biodefense pathogens

Organism

Strain

PCR Assay Number

23

28

29

30

09

11

14

15

49

50

65

01

04

07

F. tularensis

239

0.32

0.68

            

F. tularensis

240

0.52

 

0.48

           

F. tularensis

241

0.48

  

0.52

          

Y. pestis

113

    

0.21

0.61

 

0.18

      

Y. pestis

114

    

0.15

 

0.76

0.09

      

B. mallei

164

              

B. pseudomallei

197

         

0.90

0.10

   

B. anthracis

708-gi

           

0.42

0.55

0.03

B. anthracis

708-live

           

0.43

0.53

0.04

  1. Mapped read abundances (range 0 to 1) per assay amplicon for each spiked organism. Reads summed across conditions, concentrations, and replicates
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BMC Genomics

ISSN: 1471-2164

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