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Fig. 6 | BMC Genomics

Fig. 6

From: Transcriptome analysis of fungicide-responsive gene expression profiles in two Penicillium italicum strains with different response to the sterol demethylation inhibitor (DMI) fungicide prochloraz

Fig. 6

qPCR validation of 19 prochloraz-responsive DEGs including drug transporter genes (a), ergosterol biosynthesis-related genes (b), MAPK signaling pathway genes (c), and Ca2+ signal transduction genes (d). The housekeeping gene β-actin was used as internal reference to calculate the relative mRNA abundance for the selected unigenes. Relative ratios for the expression of each selected DEG were calculated as Pi-R (I/NI), Pi-S (I/NI), I (Pi-R/Pi-S), and NI (Pi-R/Pi-S). All values obtained in the qRT-PCR analysis were expressed as the mean ± SD from 5 biological repeats each containing 3 technical replicates, and independent samples t-test (n = 5) was applied in the SPSS Statistics 17.0 context to assess the significance of differences between the means (*p < 0.05 and **p < 0.01)

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BMC Genomics

ISSN: 1471-2164

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