Transcriptome analysis of fungicide-responsive gene expression profiles in two Penicillium italicum strains with different response to the sterol demethylation inhibitor (DMI) fungicide prochloraz

Table 4 Summary of GO-enriched DEGs associated with prochloraz resistance

GO ID (term)	DEG ID	DEG name (GO-annotated)	Fold change (log2) of DEG expression in the comparison below
			Pi-R (I/NI)	Pi-S (I/NI)	I (Pi-R/Pi-S)	NI (Pi-R/Pi-S)
GO:0016020 (membrane)	PITC_032590	ABC1	7.746	/	7.523	/
	PITC_006400	ABC2	1.393	/	/	−1.385
	PITC_098100	MFS1	3.880	/	1.838	−1.709
	PITC_012240	MFS2	3.853	/	/	−3.011
	PITC_056240	MFS3	2.602	/	1.922	/
	PITC_091150	MFS4	2.069	−1.512	1.225	−2.336
GO:0055114 (oxidation-reduction process)	PITC_083360	CYP51A	2.865	2.425	/	/
GO:0006694 (steroid biosynthetic process)	PITC_027000	ERG2	2.002	/	1.422	/
GO:0006694 (steroid biosynthetic process)	PITC_014340	ERG6	1.757	/	1.365	/
GO:0016301 (kinase activity)	PITC_088710	Mkk1	2.603	/	2.143	/
	PITC_062470	Hog1	/	−1.215	1.801	/
	PITC_087700	CaMK1	2.402	/	/	−1.237
	PITC_025800	CaMK2	1.138	−1.752	2.578	/
GO:0005509 (calcium ion binding)	PITC_033750	EF-hand1	1.510	/	1.543	/
GO:0005509 (calcium ion binding)	PITC_036760	EF-hand2	−1.814	/	−1.504	/

The abbreviated names for the selected DEGs are listed as: ABC1, CDR ABC transporter 1; ABC2, ABC transporter 2 (integral membrane type); MFS1, major facilitator superfamily protein 1; MFS2, major facilitator superfamily protein 2; MFS3, major facilitator superfamily protein 3; MFS4, major facilitator superfamily protein 4; CYP51A, cytochrome P450 51A; ERG2, ergosterol biosynthesis methyltransferase 2; ERG6, ergosterol biosynthesis methyltransferase 6; Mkk1, MAP kinase kinase (i.e., Mkk1,2); Hog1, MAP kinase (i.e., MpkC); CaMK1, calcium/calmodulin-dependent protein kinase 1; CaMK2, calcium/calmodulin-dependent protein kinase 2; EF-hand1, calcium-binding EF-hand 1; EF-hand2, calcium-binding EF-hand 2. Pi-R (I/NI), Pi-S (I/NI), I (Pi-R/Pi-S) and NI (Pi-R/Pi-S) indicate comparisons Pi-R-I vs Pi-R-NI, Pi-S-I vs Pi-S-NI, Pi-R-I vs Pi-S-I and Pi-R-NI vs Pi-S-NI, respectively. The log2(fold change) of DEG expression was generated from original data in DEG information lists (Table S2–5), and the slash ‘/’ indicates the unfeasibility to generate log2(fold change) when the DEG is not included in the comparison

ISSN: 1471-2164