Fig. 1

Splicing events by type for both the a Male vs Female and b Control vs Stress comparisons. Rows denote tissue type (labeled on the right), and counts of splicing events are further broken down by event type. Alternatively spliced genes in the male vs female analysis revealed, in both tissues, more events in the control versus restraint stress condition. The core exon event was the most abundant regardless of tissue or treatment. Light blue represents the control group; yellow is restraint stress. Hypothalamic retained intron events were the only event to differ significantly between treatments, represented by a red star (ChiSq p=0.012). In the control vs splicing comparison, more splicing occurred in the male hypothalamus; while in the gonad, more splicing occurred in the female. Blue represents males; green represents females. Red stars represent statistical significance between abundances in males and females, with more core exon splice events occurring in the female gonad than male (ChiSq p=3.01e-3), and more hypothalamic retained intron events found in males than females (ChiSq p=0.041)