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Fig. 4 | BMC Genomics

Fig. 4

From: Genome relationships and LTR-retrotransposon diversity in three cultivated Capsicum L. (Solanaceae) species

Fig. 4

FISH using LTR-RTs probes against metaphases and prometaphases of Capsicum species. Chromosomes were counter-stained with DAPI (blue), Copia probes with Cy3–11-dUTP (red) and Gypsy probes labelled with biotin-11-dUTP / avidin–FITC conjugate (green). The Copia Ivana/Oryco probe showed few hybridization signals scattered along chromosomes, with a low accumulated profile in both C. chinense (a) and C. baccatum (b). The Gypsy Tekay/Del probe exhibited hybridization signals dispersed along the chromosomes in the three species, but with a larger accumulation in C. annuum chromosomes (c) than the other two species, such as in C. baccatum (d). The Gypsy Athila/Tat probe showed brighter hybridization signals than Tekay/Del, accumulating in the pericentromeric to interstitial regions of all C. annuum chromosomes (e), differently of C. baccatum because some chromosomes accumulated many signals and others very few (h). The boxes i, ii, iii and iv are highlighting differences in the pericentromeric and interstitial Athila/Tat signals in two C. baccatum chromosomes. The Gypsy CRM probe showed FISH signals accumulated in the centromeric regions, but with two pairs in each species with much less intense signals. Note the arrows in C. baccatum (f) and C. chinense (g). The bar represents 10 μm

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