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Fig. 6 | BMC Genomics

Fig. 6

From: Bacillus velezensis LG37: transcriptome profiling and functional verification of GlnK and MnrA in ammonia assimilation

Fig. 6

Function authentication of LG37 with glnK deletion (ΔglnK) and overexpression (OEglnK) by RT-qPCR analyses. a Relative-expression analysis for glnK and glnL of wild-LG37 cultured with different NH4+ concentration, 5 mmol/L as control. b Quantitative relative-expression analysis of glnL, amtB, and glnA in wild-LG37 and mutant OEglnK and ΔglnK strains. c The growth curve analysis of the wild-type and both mutant strains (ΔmnrA and OEmnrA) cultured in minimum medium with 10 mmol/L NH4+. d Quantitative analysis of amtB relative expression level of wild-LG37 cultured with increasing NH4+ concentration, 5 mmol/L as the control. These results are means ± SD. *P < 0.05, **P < 0.01 versus control

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