Fig. 7

Function authentication of LG37 with mnrA deletion (ΔmnrA) and overexpression (OEmnrA) by RT-qPCR analyses. a Relative-expression analysis of mnrA gene in wild-LG37 with increasing concentration of NH₄⁺ in minimal medium, 5 mmol/L as control. b The OEmnrA expression was increased and the ΔmnrA was hindered compared to that of wild-LG37 when cultured in minimal medium with 10 mmol/L NH₄⁺. c The growth curve analysis of wild-LG37 and both mutant strains (ΔmnrA and OEmnrA) cultured in minimum medium with 10 mmol/L NH₄⁺. d The downstream functional genes glnA and glnB expression was increased in OEmnrA and decreased in ΔmnrA which cultured with 10 mmol/L NH₄⁺. These results are means ± SD. *P < 0.05, **P < 0.01 versus control