Fig. 4
Phylogenetic tree of the RND transmembrane proteins (component A of the CBA transport system) from Cupriavidus neocaledonicus STM 6070 (in bold) and from sequenced genomes of reference Cupriavidus (C.), Escherichia (E.), Legionella (L.), Microbacterium (M.), Pseudomonas (P.), Ralstonia (R.) and Xanthomonas (X.) and other named reference strains. The HME class of the protein is designated according to the current classification scheme [45, 59, 60]. HME1 to 5 represent five classes of HME systems, HAE represents here an RND protein group involved in in export of hydrophobic and amphiphilic compounds. The evolutionary history was inferred by the Neighbor-Joining method with a bootstrap consensus tree inferred from 500 replicates. The evolutionary distances were computed using the Poisson correction method and are presented as the number of amino acid substitutions per site. The rate variation among sites was modelled with a gamma distribution (shape parameter = 1). Evolutionary analyses were conducted in MEGA6. STM 6070 HME locus tags are displayed in Fig. 2 and Table S6. GenBank accession numbers or locus tags are given in brackets. For C. metallidurans CH34T only the gene numbers of the annotated genome (NC_007973) are given