Fig. 6

Application of 1RS-specific Kompetitive allele-specific PCR (KASP) marker SWK5282 and 2RL-specific KASP marker SWK252224. a Using the 1RS-specific KASP marker SWK5282, the partial genotyping results of 1RS translocation lines in 161 wheat cultivars/lines were displayed. Orange rotund shape represents the homozygous 1RS special SNP (‘Allele1/Allele1’) indicating rye Imperial, blue square shapes represent the homozygous wheat special SNP (‘Allele2/Allele2’) indicating Chinese Spring and the wheat culitivars/lines without 1RS translocations, and green triangle shapes represent the presence of both Allele1 and Allele2 indicating the wheat cultivars/lines containing 1RS translocations. Black diamond shapes indicate no template control. b Using the 2RL-specific KASP marker SWK252224, 2RL chromosomes in 81 F₂ individuals of WR91 and Shixin633 were identified. Orange rotund shape represents the homozygous 2RL special SNP (‘Allele1/Allele1’) indicating rye Imperial, blue square shapes represent the homozygous wheat special SNP (‘Allele2/Allele2’) indicating Chinese Spring and F₂ individuals without 2RL chromosome, and green triangle shapes represent the presence of both Allele1 and Allele2 indicating F₂ individuals containing 2RL chromosome. Black diamond shapes indicate no template control