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Fig. 1 | BMC Genomics

Fig. 1

From: Genome-wide analysis of MicroRNA-messenger RNA interactome in ex-vivo gill filaments, Anguilla japonica

Fig. 1

Hypertonic stress induces differential gene expression of osmotic stress transcriptional factor and seawater ion transporter. Eel gill filaments were challenged in hyperosmotic (Hyper: 600 mOsmol l− 1) media for 4, 8 and 24 h. Control (Ctrl: 300 mOsmol l− 1) or hypertonic-treated gill filaments were subsequently processed for analysis of tonicity-responsive gene expression by quantitative SYBR Green Real-Time PCR (qRT-PCR). Differential gene expression of osmotic-stress transcriptional factor (ostf1), Na+/K+ exchange regulatory cofactor (nherf1), with-no-lysine kinase 1 (wnk1), inward rectifying K+ channel (kir), cystic fibrosis transmembrane conductance regulator (cftr) and Na+/Cl-taurine transporter (taut) was determined. Gapdh expression was used as internal control for normalization of target gene expression. Results (mean ± s.e.m.) were from five independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001 vs control

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