Fig. 6

Inhibition miR-29b-3p/miR-200b-3p in primary gill cell culture. To determine the biological effects of miR-29b-3p and miR-200b-3p through loss-of-function, two specific miRNA inhibitors (miR-29b-3p or miR-200b-3p) were transfected in gill cell culture. a qRT-PCR was performed to determine the efficiency of inhibition on miR-29b-3p and miR-200b-3p. Venn diagram shows the overlay of up-regulated genes in 4/8-h hypertonic (Hyper) treatment and b miR-29b-3p inhibition, and c miR200b-3p. The figures in parentheses represent the numbers of co-expressed genes. The potential target genes of miR-29b-3p and miR-200-3p were listed in the table. d Using miRanda algorithm, the full length of the miR was aligned against the target gene sequences. Thermodynamic stability of the RNA duplexes is based on these alignments and strict alignment in the seed region at position 2–7 bp of the miR to the mRNA sequence. * P < 0.05 vs control