Fig. 3

Testing three media transfer sequences for efficiency of incorporation of the template DNA. a Three sequences of media transfer tested on the GFP collection strain Htb2-GFP following transformation with the plasmids indicated in (b). Cells were washed twice with water between each media transfer, and incubation periods at each step are indicated. b Colonies were counted following plating of fixed numbers of cells onto SC 5-FOA and SC 5-FOA G418. Proportions represent the number of colonies formed on SC 5-FOA G418 compared to SC 5-FOA, indicating that they have integrated the RFP template plasmid, which confers G418 resistance. Results from each of the three methods in (a) are shown. The mean of 3 biological replicates is shown for each method and error bars represent standard deviation