Table 2 Annotation of nuclease-independent, ITR-Seq-identified off-target sites to genomic location

AAV8-SaCas9 + AAV8-sgRNA-ctrl

Mouse A

2:98666941–98666946

5

Gm10800

AAV8-SaCas9 + AAV8-sgRNA-ctrl

Mouse B

5:90465978–90465995

8

Alb

AAV8-LbCpf1 + AAV8-sgRNA-ctrl

Mouse A

–

–

–

AAV8-LbCpf1 + AAV8-sgRNA-ctrl

Mouse B

2:98667196–98667216

29

Gm10800

2:31519035–31519051

22

Ass1

5:90474802–90474819

3

Alb

AAV8-AsCpf1 + AAV8-sgRNA-ctrl

Mouse A

–

–

–

AAV8-AsCpf1 + AAV8-sgRNA-ctrl

Mouse B

2:31518461–31518481

106

Ass1

5:90472660–90472675

7

Alb

9:103219199–103219216

3

Trf

AAV8-SaCas9 + AAV8-sgRNA-ctrl

Mouse A

–

–

–

AAV8-SaCas9 + AAV8-sgRNA-ctrl

Mouse B

2:98666940–98666966

67

Gm10800

5:90476386–90476411

12

Alb

5:90472060–90472079

11

Alb

9:21837574–21837595

10

Dock6

9:69244457–69244483

5

Rora

5:90505454–90505480

4

Alb

AAV8-LbCpf1 + AAV8-sgRNA-ctrl

Mouse A

–

–

–

AAV8-LbCpf1 + AAV8-sgRNA-ctrl

Mouse B

–

–

–

AAV8.EGFP (rhesus macaque)

d7 - 1w

6:157029767–157029776

12

–

AAV8.EGFP (rhesus macaque)

d35 - 1w

2:7161434–7161476

104

AAV8.EGFP (rhesus macaque)

d45 - 1 m

–

–

–