Table 6 Primers of DEmRNAs
Gene name | Forward primer (5′-3′) | Reverse primer (5′-3′) | Product length/bp | Annealing temperature | mRNA version |
|---|---|---|---|---|---|
3BHSD | agggcatctcagtggtca | ggataaagactggcacgcta | 144 | 57.4 °C | NM_001285716.1 |
LEPR | ccattgagaagtatcagttcagtc | catgctggtgtttttcatcatcttg | 105 | 58.4 °C | XM_018045220.1 |
STAR | cagaagggtgtcatcagagc | tgagcagccaggtgagttt | 97 | 58.6 °C | XM_013975437.2 |
CCL21 | ccgaaagaagattcccgcca | ggcgagaacaggatagctgg | 90 | 60.1 °C | XM_005684096.3 |
RARRES1 | gcgcgtgggttaatcagaag | acattaacagctggtctgggtt | 148 | 59.8 °C | XM_018048385.1 |
DPT | gtaccagacatgctccaacaa | ctgttgtcagccagcaggaa | 137 | 59.4 °C | XM_005690613.3 |
β-actin | tgcttctaggcggactgatt | tacaatcaaagtcctcggccac | 106 | 59.7 °C | NM_001314342.1 |