Fig. 9From: Whole-genome sequencing of Puccinia striiformis f. sp. tritici mutant isolates identifies avirulence gene candidatesA schematic procedure for variants calling and annotating, association analysis and effector evaluation. Variants calling and annotating were implemented mainly using GATK HaplotypeCaller and SnpEff, respectively. The output files were integrated with phenotypic data to fulfill association analyses using GAPIT. The Avr effector candidates obtained from selected associated proteins were further evaluated and characterized in different aspectsBack to article page