Fig. 1

Identifying syntenic homologs and isolating split-gene candidates. a Homology classifications from syntenic homology pipeline. b Schematic for calculation of tandem duplicate percentage. We require the ratio of L1 to L2 to be < 0.1 (i.e. the proportionate overlap of the BLAST query genes with respect to the total aligned space of the subject gene). c Summary of homology classifications and split-gene candidate filtration. A ‘Testable candidate’ is one in which all of the genes involved are expressed. d Corroboration of testable candidates. E.g. 43 ‘Corroborated’ split-gene candidates in the B73 annotation (‘B73 - Split’) were simultaneously identified as a single gene in W22 and PH207, while there were 61 genes in B73 that corresponded to multiple genes in both PH207 and W22 (‘B73 - Merged’), and the 438 ‘Unique’ split-gene candidates in B73 were identified as a single gene in W22 or PH207