Fig. 1
Experimental design and transcriptome information. a Experimental design. Sea cucumber coelomocytes isolated from H. leucospilota were challenged with LPS (10 μg/ml), Poly (I:C) (10 μg/ml) and V.harveyi (107 cell/ml) for 24 h with three biological duplicates. b The length distribution of all-Unigene. The X-axis represents the sequence size, and the Y-axis represents the number of Mix-Unigene. The orange bar shows the number of unigene which is the representative sequences, and the blue bar shows the number of transcripts which include the rough sequences. c Venn diagram of Unigene annotation. The databases used for gene annotation include NR、KOG、KEGG、SwissProt and InterPro. d Species distribution of Unigene annotation in NR database