Table 6 Primers designed for RT-qPCR of DEGs
Gene ID | P-values | Forward primer sequences (5′-3′) | Reverse primer sequences (5′-3′) |
|---|---|---|---|
Differentially expressed genes(DEGs) | |||
 CL3876.Contig4_Mix | 0 | TCACGGCATTGAGAACGG | CGGTGTCTGAACTTTGGGT |
 Unigene38924_Mix | 0 | CAGACAAAAGCCTCAGAAATG | AAGAACCCAGAGCTGGAAAG |
 Unigene36248_Mix | 0 | ATACGAAAAGCGGCAAACA | TCCATCTCAGACCCCAACTC |
 Unigene1474_Mix | 0 | TCCATCTCAGACCCCAACTC | TTTCCATTGGGAGGTAAGC |
 Unigene6464_Mix | 0 | TCACTGCGGCATTGATTC | GGTGCCCTTCCGTCAATT |
 Unigene36250_Mix | 0 | GTGCAAAGAAGGGAAGGC | TCTGGTGATTTATGTTGTGGG |
 Unigene42438_Mix | 0 | ACTCATGTGGATGGCACTAGC | GGAAGAAGGGACAGAATAAACC |
 CL3998.Contig1_Mix | 1.89E-260 | AAGGATTATGGACGGGACAC | TTCTCAAACCACCAGAGGG |
 Unigene1910_Mix | 0 | GTCAAACCAATAACAGCGAAAG | TGGACCAGACCAGGCAAA |
 Unigene27584_Mix | 0 | CGTGCTTGCCGAGTTTGT | GCAGGCTATTGCTGTTGTTAA |
 CL657.Contig6_Mix | 0 | AAGCAAGATAGACACTGTGGTTC | GATTGATGCCCCGTAAGC |
 CL3619.Contig1_Mix | 0 | AGGGTAACAGGTTGGGTGAA | GCAGTAGTTGGAGCCATTGAA |
 Unigene11374_Mix | 0 | TGCGTTTGTTGACTTTTGC | TGAAATGGTTCCGATTCTCA |
 Unigene2830_Mix | 0 | TTTGCTGGAGTATTGTGGTTC | TGGGCTGTAGGGGCTTTA |
 Unigene6841_Mix | 2.03E-139 | ACCCCAGATGACCTTTGACC | GTTAACACAATTTCTCCAGTTAGGA |
 Unigene2638_Mix | 1.05E-297 | AGTTGATATTCACCAGCCTTGC | CCACTTTCCATGAAACCGTAA |
 Unigene1366_Mix | 2.56E-93 | GGAGGTCAATGGTCATTTGG | ACCCCTGGTGACCCTACAT |
 Unigene1518_Mix | 7.31E-233 | AAACACGGTATCTCCAGAATGA | AATGACCTTTGACCTCCACC |
 Unigene16828_Mix | 0 | TTCAAAAGCAGTGCCAACG | CATTATCATCATCTTGCCGAAC |
 Unigene3853_Mix | 2.50E-62 | CCACTTGGACAGCAGTGAAG | TGATGACCTGGTTTGAGAAGAC |
Cytokine genes | |||
 CCL3-X3 | 0.000883536 | CGCAAAGGATTGTGATGGT | ACAGGAAGAGGCTATACAGACG |
 CLL10 | 0.013152297 | CTTACTGGGTTGGTAGTTTTGAC | GTGCGTTCTTGCCATTCTC |
 CLL7A | 0.00023473 | TCTTGCTTCTCACCCGATTT | CCGTAGGGGACACCTCTTT |
 CLL9-X3a | 5.57E-09 | TGGTGGAATAGTGGAGGGTG | GACTGGTTGGTTGAGGCTGT |
 CLL9-X3b | 0.003436624 | AACAGACGGACAGTCATACAGC | TGGTTGAGCCCTTGATAGTG |
 CLL9-X3c | 0.005767319 | CATTCCAGTTGGTGATGGC | TGATTTCCTCACCGCATTT |
 CLL9-X3d | 2.23E-07 | CAGAAAGCCTGGGTAAAGC | GACCTGGACCAAACCTCATT |
 EDRF1a | 0.101229003 | TTACAAGACTATCCTCCCCTCA | GCGATGCGATACTGATAAAGG |
 EDRF1b | 0.311031428 | ATGGCTTTTGAGGATGGTG | GCTGCTGTATCTCAGGAGGAAA |
 EDRF1c | 0.000470648 | ACCAACCACCATCCAACTG | TTCTCCTGATCCACCCCTAA |
 EDRF1d | 0.001415506 | TCCCTTTATTGTTCCTGTGG | TGTCTCCGCTTTCCTGTAGTA |
 IL-17 | 1.94E-07 | AGGCTGGCTCGCTTCTT | ACGCTATCGCTCATTGGAC |
 IL-17-2 | 5.40E-08 | GTTGAGGCATTCACATTTCG | AACTTTCGGCAGTCCTTACAG |
 IL-17B | 1.36E-05 | TACCATAGGAAGGGGATCTGT | AGACTTGCCCGACGATTT |
 IL-17C/E | 3.56E-23 | AGGCACTCTTCTGCATTCTCC | GCTACTGAACGCTTGTTGACG |
 TPO-I-7A2 | 1.90E-07 | CGGCGGCATACAACAGA | ACATTGGGCATCCAGAACA |
 TPO-I-7A3 | 0.003209029 | GGTCCAAAGAAAGGCATCA | GGCTTGAAAGTAAGAAACCGT |
 TPO-I-7B1 | 5.20E-08 | GATTCAGCCTTCATTTTGGA | GCTTAGTATCTTTGGGTCTGTCTA |
 TPO-I-7B2 | 0.000343861 | GATTCAGCCTTCATTTTGGA | GCTTAGTATCTTTGGGTCTGTCTA |
 TPO-I-7B3 | 0.7252968 | TGGTCCAAGTGGTCTCCCT | CTGGTCCTCTGGTAAACAGTCAT |
 TSP-4 | 0.086692271 | CAGGAGTCTTATTTGGTATCTATGC | CAATGTGGCGATGGTGTTC |