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Fig. 3 | BMC Genomics

Fig. 3

From: Genome-wide analysis of Myo-inositol oxygenase gene family in tomato reveals their involvement in ascorbic acid accumulation

Fig. 3

Ascorbate concentration in leaf and red ripe fruits of transgenic MIOX4 tomato lines and relative expression of AsA biosynthesis, recycling-related genes. a. Ascorbate content in young leaf. b. Reduced and total ascorbate contents in red ripe fruits. c. Relative expression of AsA biosynthesis and recycling-related genes in leaf. GPI (glucose-phosphate isomerase, Solyc04g076090), PMI (phosphomannose isomerase, Solyc02g086090), PMM (phosphomannomutase, Solyc05g048760), GMP1 (GDP-Man pyrophosphorylase, Solyc03g096730), GMP2 (GDP-D-mannose pyrophosphorylase 2, Solyc06g051270), GMP3 (GMP synthase, Solyc07g006090), GMP4 (GDP-Man 4 6-dehydratase, Solyc03g118270), GME1 (GDP-Man-3′,5′-epimerase 1, Solyc01g097340), GME2 (GDP-Man-3′,5′- epimerase 2, Solyc04g077020), GPP1 (L-Gal 1-phosphate phosphatase 1, Solyc04g014800), GGP (GDP-L-Gal phosphorylase/L-Gal guanylyltransferase, Solyc06g073320), GalDH (L-Gal dehydrogenase, Solyc01g106450), GLDH (L-GalL dehydrogenase, Solyc10g079470), MDHAR (monodehydroascorbate reductase, Solyc09g009390), DHAR1 (dehydroascorbate reductase, Solyc05g054760), MIOX (myo-inositol oxygenase, Solyc12g008650), AO (ascorbate oxidase, Solyc04g054690), cAPX (Cytosolic ascorbate peroxidase, Solyc06g005160), tAPX (thylakoid ascorbate peroxidase, Solyc11g018550), AOBP (ascorbate oxidase promoter-binding protein, Solyc02g088070). Three replicate experiments were performed. Error bars represent standard error, means ± SE. FW, fresh weight. The asterisks represent significant differences from wild-type (Alisa Craig), as indicated by the t-test (*P < 0.05; **P < 0.01)

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