Genomic analysis of a riboflavin-overproducing Ashbya gossypii mutant isolated by disparity mutagenesis

BMC Genomics

Table 1 Homozygous mutations of genes in MT strain

Chromosome	Position	WT seq.	MT seq.	Quality	Mutation	Gene	Product	DNA changes	Protein changes	Number
Chromosome	Position	WT seq.	MT seq.	Quality	Mutation	Gene	Product	DNA changes	Protein changes	WT seq.	MT seq.
II	496,139	C	T	1495.42	missense	AGOS_ABR055C	Transcriptional activator (AgSOK2 or AgPHD1)	c.1180G > A	G394R	0	38
III	726,948	CG	C	1167.38	frameshift	AGOS_ACR215C	Cytosolic serine hydroxymethyltransferase (AgSHM2)	c.1332delC	p.Q445fs	0	30
IV	1,433,004	T	A	1442.42	missense	AGOS_ADR404C	Oleate-activated transcription factor (AgOAF1 or AgPIP2)	c.2317A > T	p.T773S	0	38
IV	1,433,040	T	G	1455.42	missense	AGOS_ADR404C	Oleate-activated transcription factor (AgOAF1 or AgPIP2)	c.2281A > C	p.T761P	0	39
IV	199,365	G	A	1523.42	missense	AGOS_ADL287C	Chorismate synthase (AgARO2)^a	c.206C > T	p.T69M	0	39
V	70,024	C	A	1836.42	missense	AGOS_AEL305C	Large subunit of acetohydroxyacid synthase (AgILV2)^a	c.1365G > T	p.Q455H	0	46
VII	791,717	C	A	1505.42	missense	AGOS_AGL123W	Cytidine deaminase (AgCDD1)	c.314C > A	p.P105Q	0	41
VII	962,069	G	A	1560.42	nonsense	AGOS_AGL036C	Heat shock protein 104 (AgHSP104)	c.1066C > T	p.Q356*	0	42
VI	1,753,850	G	A	1884.42	missense	AGOS_AGR382W	L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase (AgLYS5)	c.365G > A	p.R122H	0	49

These homozygous mutations are a subset among all 32 homozygous mutations which are shown in Table S1
^aFlavoproteins
*Translation stops here

ISSN: 1471-2164