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Table 5 Heterozygous mutations in genes involved in sporulation

From: Genomic analysis of a riboflavin-overproducing Ashbya gossypii mutant isolated by disparity mutagenesis

ChromosomePositionWT seq.MT seqQualityMutationGeneProductDNA changesProtein changesRead numberMT seq ratio
WT seq.MT seq.
III41,510TG1253.19missenseAGOS_ACL181C1,3-beta-D-glucan synthase (AgFKS1 or AgGSC2)c.4596A > Cp.Lys1532Asn23370.617
II101,053TG878.19missenseAGOS_ABL159WComponent of the septin ring (AgSHS1)c.1229 T > Gp.Ile410Ser20290.592
IV649,360GA886.19missenseAGOS_ADL029WComponent of the meiotic outer plaque of the spindle pole body (AgSPO74)c.374G > Ap.Ser125Asn19260.578
V965,295AACAG1023.15disruptive_inframe_insertionAGOS_AER177WTranscription factor targeting filamentation genes (AgTEC1)c.1518_1520dupGCAp.Gln507dup21280.571
IV1,263,702GT982.19missenseAGOS_ADR317CDual-specificity kinase (AgMPS1)c.2228C > Ap.Thr743Asn24310.564
VI1,158,992AG1022.19missenseAGOS_AFR400CN-formyltyrosine oxidase (AgDIT2)c.635 T > Cp.Ile212Thr25320.561
III48,243CA765.19missenseAGOS_ACL179CMeiosis-specific protein (AgSPO77)c.1601G > Tp.Arg534Ile18230.561
VI1,225,184AC753.19missenseAGOS_AFR436CComponent of the septin ring (AgCDC11)c.371 T > Gp.Val124Gly21250.543
I436,519AAT935.15frameshiftAGOS_AAR053W1,3-beta-D-glucan synthase (AgGSC2 or AgFKS1 or AgFKS3)c.916_917insTp.Arg306fs24280.538
VI1,531,918TC659.19missenseAGOS_AFR604CComponent of the meiotic outer plaque of the spindle pole body (AgSPO21)c.2531A > Gp.Gln844Arg18200.526
VI1,288,694GT943.19missenseAGOS_AFR469Wt-SNARE protein (AgSEC9)c.918G > Tp.Glu306Asp27290.518
VI672,483CT645.19missenseAGOS_AFR130WProtein involved in the control of meiotic nuclear division (AgSSP1)c.121C > Tp.Leu41Phe20210.512
IV1,456,401GT500.19missenseAGOS_ADR416WMitotic exit network scaffold protein (AgNUD1)c.1263G > Tp.Gln421His16160.500
VI639,702CT610.19missenseAGOS_AFR111CComponent of the septin ring (AgCDC3)c.203G > Ap.Gly68Asp22220.500
VII1,087,176GA676.19missenseAGOS_AGR031WTranscriptional repressor (AgNRG1 or AgNRG2)c.107G > Ap.Ser36Asn25240.490
VI1,225,449AT596.19missenseAGOS_AFR436CComponent of the septin ring (AgCDC11)c.106 T > Ap.Ser36Thr25210.457
V1,436,486GA466.19missenseAGOS_AER416CEH domain-containing protein (AgEND3)c.4C > Tp.Pro2Ser21170.447
VI566,815AC758.19missenseAGOS_AFR076WSerine/threonine protein kinase (AgIME2)c.1142A > Cp.Tyr381Ser30240.444
IV1,423,888GA643.19missenseAGOS_ADR400WGamma-tubulin small complex receptor (AgSPC72)c.278G > Ap.Ser93Asn24190.442
IV650,376AT523.19missenseAGOS_ADL029WComponent of the meiotic outer plaque of the spindle pole body (AgSPO74)c.1390A > Tp.Ile464Phe26190.422
VII394,750GA560.19missenseAGOS_AGL162CSm-like protein (AgSEC1)c.1972C > Tp.Pro658Ser26190.422
III506,687TG578.19missenseAGOS_ACR083CMeiosis-specific component of the spindle pole body (AgDON1 or AgCUE5)c.237A > Cp.Arg79Ser28180.391
III38,740CA576.19missenseAGOS_ACL182C1,3-beta-glucanosyltransferase (AgGAS2)c.1161G > Tp.Glu387Asp40210.344
VI1,794,575AC577.19missenseAGOS_AFR736CTranscription factor required for response to pheromones (AgKAR4)c.422 T > Gp.Phe141Cys46210.313