Fig. 2From: On the utility of RNA sample pooling to optimize cost and statistical power in RNA sequencing experimentsVariance at different pool sizes. The variance of the gene expression levels from pooled and non-pooled experiments. In particular, the virtual counts Uj were generated from a negative binomial distribution with mean μj and over-dispersion parameter ϕ. \(\mu _{j} = \rho L_{j}^{0}\), where ρ is the relative abundance (ρ=10−6), and \(L_{j}^{0}\) is the virtual library size in biological sample j, and \(L_{j}^{0}\) are uniformly sampled between 15−25×106. Yk is the outcome from a pooled design with a pool of size q according to the model in (1)Back to article page