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Fig.4 | BMC Genomics

Fig.4

From: Protein changes as robust signatures of fish chronic stress: a proteomics approach to fish welfare research

Fig.4

a – Volcano plots of the entire set of plasma proteins detected by DIGE analysis on the NET trial samples. Each point represents the difference in abundance (fold-change) between stressed fish (NET2 on the left; NET4 on the right) and control fish plotted against the level of statistical significance. Dotted vertical lines represent a 2-fold variation in abundance, while dotted horizontal line represent the significance level of P < 0.05. Red dots represent proteins significantly up- and down-regulated. b – Principal component analysis performed with the normalized spot volumes of the 107 identified proteins in the plasma samples of gilthead seabream from the NET trial (n = 6). Blue, orange and red dots represent CTRL, NET2 and NET4 groups, respectively. c – Hierarchical clustering of 107 significantly differential proteins identified in the plasma samples of gilthead seabream from net handling (NET) trial. Rows represent expression patterns of individual proteins, while each column corresponds to a biological replicate (fish). Cell colour indicates the normalized Z-scores of the spot volumes

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