Fig. 1

Starvation explains the lifespan effect of H₂O₂ food (a) The correlations between mean lifespan of 31 DGRP lines on food containing either 2% glucose and 2% hydrogen peroxide in replicate trials (peroxide 1 and peroxide 2) or no glucose (starvation). Below the diagonal are plots of trait values (mean survival (h)). Least-squares linear regression lines are shown in red. Above the diagonal are Spearman correlation coefficients for each pair of traits. b and c Feeding assays, the mean (+SD) absorbance at 630 nm of extracts from three replicate vials of seven to eleven 1 to 5 day old mated females from the indicated DGRP line (b). Flies were exposed to peroxide (open bars) or control food (colored bars) for two hours of feeding prior to dye extraction. c The mean (+SD) volume of liquid food consumed by ten replicates of ten 2 to 4 day-old mated females was measured using the CAFE assay. CAFE food contained 2% glucose and, either water (colored bars) or 2% peroxide (open bars). Additional apparatus were set up without flies (no flies) to measure volume loss due to evaporation. Asterisks indicate p < 0.05 (Welch’s t-test)