Table 2 Oligonucleotide primers used in this study
Primer Name | Sequence (5′-3′) | Reference |
|---|---|---|
Primers used for making knockout mutants | ||
 atpBC_F | ATGGCTGCAGGAGAAATCTCTACGCCGCAAGAGTACATAGGTCATCATCTGTGTAGGCTGGAGCTGCTTC | This study |
 atpBC_R | TTACATCGCGTTTTTGGTCAACTCGATCACGCGCAGTTTGGCGATCGCTTCATATGAATATCCTCCTTA | This study |
 atpB_R | TCAATGTTCTTCAGATGCCATCGACAGATAGACAATCGTTAAGACCATGACATATGAATATCCTCCTTA | This study |
Primers used for the overexpression construct of pspA | ||
 pBAD_FC_F | GGCTGTTTTGGCGGATGAGA | This study |
 pBAD_FC_R | AATTCGCTAGCCCAAAAAAACGG | This study |
 pspA_FC_F | CCGTTTTTTTGGGCTAGCGAATTATGGGTATTTTTTCACGTTTTGCCG | This study |
 pspA_FC_R | TCTCATCCGCCAAAACAGCCTTATTCACCGATACGCCGGTT | This study |
Primers used for confirming knockout of genes | ||
 atpBC_CF | CCGGCTGTAATTAACAACAAAG | This study |
 atpBC_CR | TTTCCTGACTGGCCTTCT | This study |
 atpB_CR | CCATGTACAGCAGATCCATATT | This study |
Primers used for the transcriptional fusion construct of pspA | ||
 pspA_tsc_F | CGACCTGAATGGAAGCCGGCGCCAGTTCGTCGAGAAACAAC | This study |
 pspA_tsc_R | GAGCTCGGTACCCGGGGATCCTCAATCAAATTCCTCATCAGTCTGG | This study |
 pPROBE-NT_tsc_F | GAGGATCCCCGGGTACCGAGCTC | [27] |
 pPROBE-NT_tsc_R | GCCGGCTTCCATTCAGGTCG | [27] |
Primers used for qRT-PCR | ||
 qhok_F | TGGTGCGTACTTATAGTGTGTG | This study |
 qhok_R | CCGGATTCGTAAGCCATGAA | This study |
 qpspA_F | GACCTGATTGCTGCTTTGC | This study |
 qpspA_R | GGTTTCAGCCAGTTTACTTTCC | This study |
 qatpG_F | GTCGGCTATCTGGTCGTATCT | This study |
 qatpG_R | GCCTTTATCAGCCCAGGATTT | This study |
 qkatA_F | CGCGACTGGGTGTTAACTATAA | This study |
 qkatA_R | TGGATCAGAGGCAAGATCAATAC | This study |
 qhrpA_F | AGCACTTCAGCATCCAAGAC | This study |
 qhrpA_R | CGAGTTCTGCGTATCCATCTTC | This study |