Table 4 Summary of the in vitro G4 formation analyses by CD spectroscopy and ThT fluorescent assay in vitro. Sequences are shown in the 5′ to 3′ direction; all oligonucleotides are RNA. For G4 formation by CD, “Yes” indicates that a CD signature typical of a parallel G4 structure in the presence of K+. The result of CD spectroscopy was considered positive in the case of a blue-shift of the positive ellipticity peak (from 270 to 264 nm) and a stronger signal in the presence of K+ ions. Ratio between ThT fluorescence in the presence of oligonucleotide and background fluorescence of ThT alone is presented in the last column. The light-up effect ((“Fold of ThT”) refers to fold increase in Thioflavin T fluorescence emission when the candidate sequence is added: the higher this increase, the more likely is the structure to form a G4 motif.
