Telomere length de novo assembly of all 7 chromosomes and mitogenome sequencing of the model entomopathogenic fungus, Metarhizium brunneum, by means of a novel assembly pipeline

Table 4 Percentage of protein Busco completion of protein sets generated from the long-read M. brunneum assembly predicted with various ab-initio gene prediction tools and approaches

Prediction tool	Protein BUSCO (N = 4494)	Complete Busco	Single copy	Duplicated	Fragmented	Missing	Number of predicted chromosomal genes
Augustus	96.3%	4325	4313	12	58	111	10,805
GeneMarkES	99.0%	4450	4435	15	23	21	11,284
GeneMark-ES-Native no mtDNA	99.1%	4454	4439	15	19	21	11,389
GeneMark-ES-Native with mtDNA	99.1%	4455	4441	14	18	21	11,406
GlimmerM	15.6%	704	702	2	197	3593	7529

The final gene set used for functional analysis, which was subsequently deposited in the GenBank is highlighted in bold text. Note that one predicted gene in the final gene set was found to be non-protein coding. Remarkably, ab intio gene prediction of chromosomal genes was superior in terms of BUSCO score when the mitogenome was included for training of the prediction model

ISSN: 1471-2164