Table 1 Gene description, primer sequences, amplicon length, and PCR efficiency for candidate RGs and CHS1 selection in strawberry
Gene name | Gene description | Gene ID | Arabidopsis homolog locus | E values | Primer sequence Forward (5′-3′) | Primer sequence Reverse (5′-3′) | Amplicon size (bp) | PCR efficiency (%) | Correlation coefficient (R2) |
|---|---|---|---|---|---|---|---|---|---|
RPT6A | 26S proteasome regulatory particle AAA-ATPase subunit protein 6A | FvH4_1g03980 | AT5G19990 | 0 | GGTTTTGATGGCTACAAATCGT | CCATTCATTTTCTCGGCAATCT | 188 | 100.2 | 0.998 |
RPN5A | 26S proteasome regulatory particle non-ATPase subunit protein 5A | FvH4_5g27840 | AT5G09900 | 0 | GAGGCAATTTAGACGCGCAA | GCTCAAGAATGTCAGTGGCG | 107 | 99.5 | 0.997 |
VPS32 | Vacuolar protein sorting protein 32 | FvH4_1g06720 | AT2G19830 | 6e-65 | AAAGCAACGAACATAGATGACG | CTGAACCAATTGGAGTTGACAG | 105 | 100.8 | 0.998 |
SKP1 | Subunit of SCF complex, S-phase-kinase-associated protein 1 | FvH4_1g11300 | AT1G75950 | 9e-67 | TCATTTCAGTTCTCTCCACACA | GATCATGTGCTTGATCGTCTG | 202 | 99.9 | 0.999 |
AKR2B | Ankyrin repeat protein 2B | FvH4_2g17270 | AT2G17390 | e-115 | CCCAATCCTTTTGATTTCTCGG | TGACTATCAAACTGAGGGACAC | 169 | 101 | 0.999 |
YLS8 | Yellow leaf specific gene 8 | FvH4_3g08480 | AT5G08290 | 2e-80 | TTTGCTGTCATTTACCTTGTGG | GTTGATCTTGTTGTTGTTCCCA | 146 | 100.5 | 0.997 |
ATPD | ATP synthase subunit δ | FvH4_7g01010 | AT5G13450 | 1e-66 | AGTGCCTGCAGATACTAGAAAG | CTTTCACTATTCCCTTATGCGC | 176 | 98.9 | 0.996 |
ATPE | ATP synthase subunit ε | FvH4_7g08910 | AT5G47030 | 8e-77 | TGAACTCGGCCTCAACTGAC | ACAAGGGAGCACAAAGACCA | 114 | 101.2 | 0.995 |
UBC12 | Ubiquitin conjugating enzyme E2 | FvH4_3g35650 | AT5G59300 | 2e-72 | TTATCCTGATGGGCGCGTTT | GTGACTTTCTCACGCAACGG | 241 | 101.7 | 0.998 |
UBC5 | Ubiquitin conjugating enzyme E2 | FvH4_1g16390 | AT2G36060 | 8e-76 | ACAAGAGAAGGGTTCGCCAG | AACAAAAGGCGGCAACTGAC | 117 | 99.8 | 0.994 |
UBC10 | Ubiquitin conjugating enzyme E2 | FvH4_2g35960 | AT5G25760 | 9e-87 | TGCATTTCAAGACAGGAGAGAT | TAGCCCTACAAACAGACTGAAG | 158 | 99.8 | 1.000 |
UBC16 | Ubiquitin conjugating enzyme E2 | FvH4_5g03910 | AT5G53300 | 1e-83 | TTGCTGAAGACATGTTTCACTG | TCAACAGTGAGCAAATCGAAAG | 254 | 98.9 | 0.993 |
UBC18 | Ubiquitin conjugating enzyme E2 | FvH4_7g30920 | AT5G53300 | 5e-83 | CCAAAGGTGGCATTTAGAACAA | TCCTTGCTGTTGTCTCATACTT | 225 | 99.8 | 0.999 |
UBC21 | Ubiquitin conjugating enzyme E2 | FvH4_3g40820 | AT1G64230 | 1e-83 | GATAGCCCGTATGCAGGTGG | CATCAGGGTTGGGGTCTGTC | 225 | 97.8 | 0.996 |
UBC46 | Ubiquitin conjugating enzyme E2 | FvH4_3g18500 | AT1G78870 | 1e-85 | TGCCTCTCGACCCCAAAAAT | GGGAAGGTTACTGTTCGCCA | 147 | 101.8 | 1.000 |
UBC50 | Ubiquitin conjugating enzyme E2 | FvH4_3g25890 | AT3G52560 | 1e-73 | GTGGAGAAAAGGGCATCGGA | CGCCCCTCGTGAACAGTATT | 120 | 100 | 0.997 |
UBC51 | Ubiquitin conjugating enzyme E2 | FvH4_6g19850 | AT2G36060 | 8e-76 | TCCTTCTCACTTGCCTTCGTC | AGCCTAGCGTCATGGGTACT | 121 | 100.2 | 0.998 |
EF1ɑ2 | Elongation factor 1-alpha | FvH4_7g20050 | AT5G60390 | 0 | GCTTCAAACTCCAAGGATGATC | CTTAACAAAACCAGCATCACCA | 233 | 99.6 | 0.999 |
EF1ɑ1 | Elongation factor 1-alpha | FvH4_3g33150 | AT5G60390 | 0 | ATACAACCCAGACAAAATTGCC | ACCACCGATCTTGTATACATCC | 192 | 101.6 | 0.995 |
TUA2 | Alpha tubulin like protein | FvH4_1g18660 | AT1G50010 | 0 | CTTCAACACCTTCTTCTCCGAG | GATCTCTTTGCCGATGGTGTAG | 176 | 98.8 | 0.999 |
HISTH4.1 | Histone H4 | FvH4_6g14140 | AT2G28740 | 0 | TCAAGCGTATCTCCGGTCTC | AGTGTCCTTCCCTGCCTCTT | 163 | 97.8 | 0.994 |
UBQ11 | Ubiquitin | / | CAGACCAGCAGAGGCTTATCTT | TTCTGGATATTGTAGTCTGCTAGGG | / | 98.6 | 0.995 | ||
ENP1 | Endoplasmin-like protein | / | GCCACGTCTCTTTGACATTGACT | TTCCGAATGGGCTTTCCA | 71 | 99.3 | 0.992 | ||
CHP1 | Conserved hypothetical protein | / | TGCATATATCAAGCAACTTTACACTGA | ATAGCTGAGATGGATCTTCCTGTGA | 91 | 98.9 | 0.99 | ||
ACT6 | Actin protein | FvH4_1g23490 | AT5G09810 | 0 | GCCAACCGTGAGAAGATG | TCCAGAGTCAAGAACAATACCAG | 106 | 100.8 | 0.998 |
26S–18S | 18S–26S interspacer ribosomal gene | ACCGTTGATTCGCACAATTGGTCATCG | TACTGCGGGTCGGCAATCGGACG | 149 | 100.6 | 0.999 | |||
GAPDH4.1 | Glyceraldehyde-3-phosphate dehydrogenase | FvH4_4g24420 | AT1G13440 | e-172 | TCCATCACTGCCACCCAGAAGACTG | AGCAGGCAGAACCTTTCCGACAG | 196 | 100.8 | 1.000 |
CHS1 | Chalcone synthase | FvH4_7g01160 | AT5G13930 | 0 | ACGCAACAACACACAGCTCC | TTGGGAGGAGTTGCAGTCCC | 173 | 99.1 | 0.994 |