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Fig. 1 | BMC Genomics

Fig. 1

From: Impact of short-read sequencing on the misassembly of a plant genome

Fig. 1

Identification of high, low, and background coverage regions. a Normalized read depth (RD) of 100 bp bins using reads from dataset1, dataset2, and a dataset2 subset at 30-fold coverage in regions 4.3 ~ 4.5 Mb on Chromosome 1. Black and grey arrows show regions with high read coverage and low coverage, respectively. b RD distributions of high coverage (HC), background (BG), and low coverage (LC) regions called by CNVnator. Dashed lines at RD = 0.72 and 1.76: threshold values defining the boundaries between LC and BG regions and between BG and HC regions, respectively. c, d Proportion of called regions above or below RD value thresholds distinguishing LC and BG regions (c) and distinguishing BG and HC regions (d). Vertical dashed line: threshold value

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