Table 1 Summary of injected sgRNA and Cas9 mRNA and mutants in CRISPR/Cas9-gene editing experiment. The bracket is the number of larvae and adult which showed phenotypic changes
Gene | Gene ID | Target sites | Final concentration of injected sgRNA (ng/μl)a | Injected eggs | Hatching larva (hatching rate) | L5 (Mutants) | Mutation rate in L5 (%) | Pupa | Adult (Mutants) | Mutation rate in adult (%) |
|---|---|---|---|---|---|---|---|---|---|---|
white | Px_03417_w | T_8165, T_8232, T_8700 | 990 | 245 | 76 (31.02%) | 69 (21) | 30.43 | 52 | 39 (5) | 12.83 |
scarlet | Px_03415_st | T_661, T_684 | 814 | 260 | 48 (18.46%) | 28 (0) | 0 | 24 | 11 (4) | 36.36 |
brown | Px_17845_w | T_15076, T_16066 | 925 | 485 | 119 (24.54%) | 70 (16) | 22.86 | 65 | 61 (0) | 0 |
ok | Px_17844_st | T_4354, T_4454 | 925 | 250 | 40 (16%) | 12 (3) | 25 | 10 | 9 (0) | 0 |
lightoid | Px_17846_ltd | T_2271, T_2307, T_3097, T_3154 | 800 | 260 | 98 (37.69%) | 27 (15) | 55.55 | 27 | 25 (0) | 0 |
Control | NA | NA | NA | 31 | 18 (58.06%) | 12 | NA | 10 | 10 | NA |