Skip to main content

Table 4 The average amino acid use of the top 5% expressed genes (Top5One-tissue) in G. bimaculatus and 5% lowest expressed genes for the organism-wide analyses (using average expression across all nine tissue types). The number of predicted tRNAs in the genome per amino acid are shown. SE is the standard error

From: Adaptation of codon and amino acid use for translational functions in highly expressed cricket genes

Amino acid (AA) S/C Score AA Freq. High exp SE AA Freq. Low exp SE Percent Diff. P tRNAs
Gly 1 6.66 0.21 8.71 0.13 −30.70 ** 71
Ala 4.76 7.32 0.24 11.54 0.14 −57.72 ** 75
Val 12.28 6.73 0.19 6.27 0.08 + 6.80 ** 96
Ile 16.04 5.70 0.15 2.91 0.04 + 49.01 ** 41
Leu 16.04 9.07 0.26 8.13 0.10 + 10.31 ** 141
Ser 17.86 6.75 0.21 7.63 0.11 −12.94 ** 132
Thr 21.62 5.16 0.15 5.08 0.07 + 1.69   103
Lys 30.14 6.93 0.18 3.53 0.06 + 49.08 ** 70
Pro 31.8 4.62 0.15 6.95 0.11 −50.40 ** 103
Asp 32.72 5.08 0.16 3.83 0.06 + 24.64 ** 31
Asn 33.72 4.30 0.13 2.68 0.04 + 37.70 ** 37
Glu 36.48 6.53 0.22 5.09 0.07 + 22.08 ** 49
Gln 37.48 3.75 0.15 3.49 0.05 + 6.92 * 76
Phe 44 4.10 0.10 2.70 0.04 + 34.20 ** 48
Arg 56.34 5.61 0.15 10.04 0.12 −78.95 ** 125
Tyr 57 3.10 0.08 1.87 0.05 + 39.53 ** 43
Cys 57.16 2.08 0.06 2.51 0.03 −20.75 ** 38
His 58.7 2.24 0.07 2.53 0.04 −12.99 ** 37
Met 64.68 2.61 0.06 2.32 0.02 + 10.93 ** 43
Trp 73 1.18 0.03 1.48 0.02 −25.80 ** 32
  1. Notes: A negative correlation between S/C score and the frequency of amino acids was observed for highly and lowly expressed genes (Spearman’s Ranked R = -0.87 and − 0.75, P < 10−7). Further, a positive correlation between the frequency of amino acids and tRNA counts was observed for highly and lowly expressed genes (Spearman’s Ranked R = 0.65 and 0.74, P = 2.6X10−3 and P < 10− 7). Percent Diff. = percent difference. In column with P values, * indicates P < 0.05–0.001, **indicates P < 0.001 using a two tailed t-test (α = 0.05). All P values for t-tests in the table withstand Bonferroni correction (P < 0.05 after corrected by the number of tests) with the exception of Gln