Table 4 The average amino acid use of the top 5% expressed genes (Top5_One-tissue) in G. bimaculatus and 5% lowest expressed genes for the organism-wide analyses (using average expression across all nine tissue types). The number of predicted tRNAs in the genome per amino acid are shown. SE is the standard error

Gly

1

6.66

0.21

8.71

0.13

−30.70

**

71

Ala

4.76

7.32

0.24

11.54

0.14

−57.72

**

75

Val

12.28

6.73

0.19

6.27

0.08

+ 6.80

**

96

Ile

16.04

5.70

0.15

2.91

0.04

+ 49.01

**

41

Leu

16.04

9.07

0.26

8.13

0.10

+ 10.31

**

141

Ser

17.86

6.75

0.21

7.63

0.11

−12.94

**

132

Thr

21.62

5.16

0.15

5.08

0.07

+ 1.69

103

Lys

30.14

6.93

0.18

3.53

0.06

+ 49.08

**

70

Pro

31.8

4.62

0.15

6.95

0.11

−50.40

**

103

Asp

32.72

5.08

0.16

3.83

0.06

+ 24.64

**

31

Asn

33.72

4.30

0.13

2.68

0.04

+ 37.70

**

37

Glu

36.48

6.53

0.22

5.09

0.07

+ 22.08

**

49

Gln

37.48

3.75

0.15

3.49

0.05

+ 6.92

*

76

Phe

44

4.10

0.10

2.70

0.04

+ 34.20

**

48

Arg

56.34

5.61

0.15

10.04

0.12

−78.95

**

125

Tyr

57

3.10

0.08

1.87

0.05

+ 39.53

**

43

Cys

57.16

2.08

0.06

2.51

0.03

−20.75

**

38

His

58.7

2.24

0.07

2.53

0.04

−12.99

**

37

Met

64.68

2.61

0.06

2.32

0.02

+ 10.93

**

43

Trp

73

1.18

0.03

1.48

0.02

−25.80

**

32