Fig. 1
From: One-step generation of a targeted knock-in calf using the CRISPR-Cas9 system in bovine zygotes
The CRISPR-mediated knock-in of bovine embryos by homology mediated end joining (HMEJ). We utilized the HMEJ donor template design with the green fluorescent protein reporter gene to develop a non-invasive screening method of bovine blastocysts to visualize knock-in embryos. a schematic representation of the complete template in the pUC19 plasmid (orange). Yellow starburst = gRNA target site at the H11 locus on chromosome 17 with gRNA/Cas9 ribonucleoprotein complex bound; LHA = left homology arm; SRY = sex-determining region Y; GFP = green fluorescent protein; RHA = right homology arm; kb = kilobase b day seven microinjected bovine blastocysts under bright field c a filter specific for eGFP fluorescence showing a fluorescent blastocyst, and d merge of bright field and fluorescent image