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Table 1 Summary of the experimental design

From: Transcriptome profiling of Lymnaea stagnalis (Gastropoda) for ecoimmunological research

Treatment Treatment type N Reference/control treatment
Untreated 5
Anaesthesia Handling 5 Untreated
Wounding Handling/immune challenge 6 Anaesthesia
Injection with E. coli Immune challenge 6 Wounding
Injection with M. lysodeikticus Immune challenge 6 Wounding
Injection with healthy snail tissue Immune challenge 6 Wounding
Injection with trematode-infected snail tissue Immune challenge 6 Injection with healthy snail tissue
Elevated temperature Environmental change 4 Untreated
Food deprivation Environmental change 4 Untreated
  1. Columns present treatments, treatment types, the number of exposed snails, and the specific reference/control treatment used for each treatment group when examining their effects on gene expression