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Table 1 Summary of the experimental design

From: Transcriptome profiling of Lymnaea stagnalis (Gastropoda) for ecoimmunological research

Treatment

Treatment type

N

Reference/control treatment

Untreated

5

Anaesthesia

Handling

5

Untreated

Wounding

Handling/immune challenge

6

Anaesthesia

Injection with E. coli

Immune challenge

6

Wounding

Injection with M. lysodeikticus

Immune challenge

6

Wounding

Injection with healthy snail tissue

Immune challenge

6

Wounding

Injection with trematode-infected snail tissue

Immune challenge

6

Injection with healthy snail tissue

Elevated temperature

Environmental change

4

Untreated

Food deprivation

Environmental change

4

Untreated

  1. Columns present treatments, treatment types, the number of exposed snails, and the specific reference/control treatment used for each treatment group when examining their effects on gene expression