Fig. 10

No FNSI activity was confirmed using candidate Sl2ODDs. a Schematic diagram of the reactions of eriodictyol under the action of different enzymes. Eri: eriodictyol, Diq: dihydroquercetin, Lut: luteolin, Que: quercetin. FNS: Flavone synthase, F3H: flavanone-3-hydroxylase, FLS: flavonol synthase. b UPLC analysis of in vitro enzyme reaction products of SlFNSIs. SlFLS-Diq/Eri: SlFLS uses Diq and Eri as substrates, respectively. The remaining enzymes use Eri as a substrate to verify the FNS activity