Fig. 3

Validation of RNA-seq expression results by comparison with quantitative reverse transcription-PCR (RT-qPCR) data from the same RNA samples. Comparison of Log2 fold changes of 10 genes at 12-hpi. TUB2 was used as a control that was not differentially expressed in the RNA-seq experiments. MON1 was used as an endogenous housekeeping control. C4-induced transcriptional changes were normalized to gene expression in noninduced seedlings. Columns represent Log2 fold changes from RT-qPCR between ß-est treated and mock treated samples. Numbers in parenthesis indicate induced Log2 fold changes from RNA-seq expression data (Additional file 2) for direct comparison with RT-qPCR data. Log2 fold changes of 4 of the 10 genes that were differentially expressed at 6-hpi are also shown. Error bars indicate standard deviations from three biological replicates