Table 2 The primer information of qRT-PCR
From: Immune profiles of male giant panda (Ailuropoda melanoleuca) during the breeding season
Gene | Primer (5′ → 3′) |
|---|---|
MFAP1-F | AACCGCCCATTCAGTCTACG |
MFAP1-R | ATGCCGGGCCAATCTTTCTT |
HSP90AA1-F | TGCGAGGAGCTAATCCCTGA |
HSP90AA1-R | TCGTGAATTCCCAGCTTGATGT |
PSMD7-F | TGTTGTTGGTGTGCTTTTGGG |
PSMD7-R | CATCTTCATCAAAAGGGACTGCAAA |
S100A9-F | CCAGCTCAGGGTTACCACAC |
S100A9-R | CCATCTTCCTGCGTTCCAAGT |
SOD1-F | AGAAGGAAGGTGGGCCTGTT |
SOD1-R | TGCACTGGTACAGCCTTGTGT |
CD3D -F | AGGCTCTCCAAGGCTGCAAA |
CD3D -R | TCTTCCGAGGCCAGTTTTCAC |
RPL9-F | TGCTGCGTCTACTGCGAGAATG |
RPL9-R | ATGTGATTGAAGTCCCTTCGCA |
KLRD1-F | ATCACAGAACTCCAGAAAGGCTC |
KLRD1-R | GACAGTGGAGCCATCTTCCC |
IGIP -F | GTGTGAACGTTTGTGGCTGG |
IGIP -R | CCCCCAAATTGGTCCACAGT |
SEC61B -F | GGCACTAATGTGGGCTCGTC |
SEC61B -R | TACTGGAACAGGGCCAACCTTGA |
PHF5A -F | GCCATCGGAAGACTGTGTGAAA |
PHF5A -R | CGCCCCTGGTAAGAGCCATA |
VMA21-F | AGCCGCCCGACTTCAGAAATGA |
VMA21-R | CCACCGCAACAATAGCAGCA |
GAPDH -F | GGTATGACAACGAATTTGGCTACA |
GAPDH -R | GATGGAAACAGTGGAGGTCAGGA |