Fig. 5

Relationship between the number of low frequency variants (< 5% abundance) and the number of input reads for the Nano-Q tool. If more reads are eligible to enter the full Nano-Q pipeline (after the initial steps of cleaning and size selection), more low frequency variants are detected. There was no saturation in the number of variants within range of eligible reads examined. However, as shown in text, detecting more low frequency variants did not cause significant changes in the frequency of major variants