Linear Mixed-Effect Models For Each Target Genea
|
---|
Gene
|
Intercept
|
Treatment
|
Post-hoc Test (lsmeans)
|
---|
F-value
|
p-value
|
F-value
|
p-value
|
---|
apod
|
165.5
|
< 0.0001
|
15.46
|
0.026
|
CT vs WH
|
c1ql2
|
1954.6
|
< 0.0001
|
48.87
|
0.005
|
CT vs WN, CT vs WH
|
c3
|
132.1
|
< 0.0001
|
2.53
|
0.227
| |
calm
|
1875.4
|
< 0.0001
|
84.92
|
0.002
|
CT vs WN, CT vs WH
|
camp-a
|
429.4
|
< 0.0001
|
6.02
|
0.089
| |
casp8
|
249.8
|
< 0.0001
|
16.97
|
0.023
|
CT vs WN, CT vs WH
|
cat
|
19,704.0
|
< 0.0001
|
4.54
|
0.124
| |
cirbp
|
356.3
|
< 0.0001
|
84.34
|
0.002
|
CT vs WN, CT vs WH
|
cldn3
|
739.8
|
< 0.0001
|
13.75
|
0.031
|
WN vs WH
|
ctsh
|
509.5
|
< 0.0001
|
76.82
|
0.003
|
CT vs WN, CT vs WH
|
cul3
|
1087.0
|
< 0.0001
|
2.28
|
0.250
| |
cyp1a1
|
362.9
|
< 0.0001
|
30.48
|
0.010
|
CT vs WN, CT vs WH
|
dnmt1
|
1240.3
|
< 0.0001
|
40.65
|
0.007
|
CT vs WN, CT vs WH
|
egln2
|
5750.5
|
< 0.0001
|
76.76
|
0.003
|
CT vs WN, CT vs WH
|
epx
|
245.1
|
< 0.0001
|
19.68
|
0.019
|
CT vs WN, (CT vs WH)
|
gck
|
189.4
|
< 0.0001
|
2.47
|
0.232
| |
gstt1
|
802.0
|
< 0.0001
|
18.43
|
0.021
|
(CT vs WN), CT vs WH
|
hcn1
|
225.1
|
< 0.0001
|
41.89
|
0.006
|
CT vs WN, CT vs WH
|
hif1α
|
394.9
|
< 0.0001
|
10.88
|
0.042
|
(CT vs WN), CT vs WH
|
hsp70
|
61.6
|
< 0.0001
|
3.18
|
0.181
| |
hsp90aa1
|
291.6
|
< 0.0001
|
45.56
|
0.006
|
CT vs WN, CT vs WH
|
hsp90ab1
|
255.8
|
< 0.0001
|
2.05
|
0.275
| |
hspd1
|
1795.4
|
< 0.0001
|
167.12
|
0.001
|
CT vs WN, CT vs WH
|
igfbp2b1
|
81.7
|
< 0.0001
|
1.68
|
0.323
| |
il8
|
124.1
|
< 0.0001
|
3.72
|
0.154
| |
irf2
|
403.4
|
< 0.0001
|
0.89
|
0.499
| |
jak2
|
318.8
|
< 0.0001
|
8.01
|
0.053
| |
jund
|
166.4
|
< 0.0001
|
16.66
|
0.024
|
CT vs WN, CT vs WH
|
mhcii
|
93.8
|
< 0.0001
|
1.69
|
0.322
| |
mmp9
|
76.1
|
< 0.0001
|
9.23
|
0.052
| |
nckap1l
|
126.7
|
< 0.0001
|
17.66
|
0.022
|
CT vs WN, CT vs WH
|
ndufa1
|
4303.5
|
< 0.0001
|
0.58
|
0.611
| |
ndufa4
|
93.1
|
< 0.0001
|
8.26
|
0.060
| |
pdk3
|
1163.6
|
< 0.0001
|
16.22
|
0.025
|
CT vs WN, (CT vs WH)
|
prdx6
|
1814.2
|
< 0.0001
|
158.74
|
0.001
|
CT vs WN, CT vs WH
|
rraga
|
1624.3
|
< 0.0001
|
46.90
|
0.006
|
CT vs WN, CT vs WH
|
serpinh1
|
54,283.9
|
< 0.0001
|
31.67
|
0.010
|
CT vs WN, CT vs WH
|
tapbp
|
69.2
|
< 0.0001
|
0.14
|
0.878
| |
tnfrsf6b
|
69.6
|
< 0.0001
|
4.61
|
0.122
| |
txn
|
82.9
|
< 0.0001
|
3.21
|
0.180
| |
ucp2
|
409.9
|
< 0.0001
|
147.53
|
0.001
|
CT vs WN, CT vs WH
|
|
Num DF = 1, Den DF = 18
|
Num DF = 2, Den DF = 3
| |
- a Linear mixed-effect models (LMEs) were performed for each gene individually to assess the effect of the fixed factor ‘treatment’ and included the random term ‘tank’. To investigate pairwise comparisons between the Control (CT), Warm & Normoxic (WN) and Warm & Hypoxic (WH) treatment groups the significant models were followed by lsmeans post-hoc tests with Tukey’s multiple comparisons adjustment of p-values. Significant values are marked in bold letters (p < 0.05) and trends are indicated in italics and/or in brackets (0.05 < p < 0.1)