Fig. 5

The ZNF516–12356 and ARNTL-1638 motifs and their expression. a Consensus sequence and secondary structure of the ZNF516–12356 motif with annotated protein binding sites and poly (A) signal sequences. b Agarose gel separation of RT-PCR products of ZNF516–12356 motif, generated by using primers for the RNA motif itself (Motif) and mRNA coding regions (Exon) (note: The full-length gel is in Fig. S4 [see Additional file 15]). M stands for DNA markers. Lanes containing PCR products are marked with (+) or (−), indicating the presence or absence of reverse transcriptase (RT). The two arrows next to the gel indicate bands corresponding to DNA products of the expected size. c Consensus sequence and secondary structure of the ARNTL-1638 motif. d Agarose gel separation of RT-PCR products of ARNTL-1638 motif (note: The full-length gel is in Fig. S4 [see Additional file 15]). The poly (A) signal is shown in the figure. Gel annotations are as described for b. e Real-time PCR results were performed using primers for the RNA motif (in the intron) and the exon containing the RNA motif (β-actin RNA was used as a control for constitutively expressed genes). The experiment was repeated twice and the results were similar